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KMID : 1137820080290020159
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의공학회지
2008 Volume.29 No. 2 p.159 ~ p.163
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The Differentiation of bone Marrow Stromal Cells into NP-like Cells through 3-Dimensional Co-culture System
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Kim Dong-Hwa
Kim Su-Hyang
Heo Su-Jin
Shin Ji-Won
Kim Young-Jick
Park So-Hee
Jun J.W.
Shin Jeong-Uk
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Abstract
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The goal of this study is to investigate the effect and potential of three-dimensional Co-culture of BMSCs (bone marrow stromal Cells) and NP (nucleus pulposus) Cells on the differentiation of BMSCs into NP-like Cells. The NP Cells and BMSCs were isolated and cultured from New Zealand White rabbits. The isolated NP Cells and BMSCs were prepared in different alginate beads. Those two types of beads were separated by a track-etched membrane of 3 ㎛ pore in a 6-well culture plate. No growth factors were used. In addition to these, NP and BMSC were cultured in the bead s independently for control. The number of Cells in Co-culturing system was half of those in two control groups. Proliferation and production of glycosaminoglycan (GAG) were evaluated along with histological observation. The GAG production rate(GAG contents/Cell) of Co-cultured BMSCs were much higher than that of BMSCs cultured alone. The total amounts of GAG produced by BMSCs in Co-culturing system were larger than those produced by BMSCs in control group and were comparable with those produced by NP alone even the number of each Cell was half of BMSCs in Co-culturing system. This study showed the potential of differentiation of BMSCs into NP-like Cells through three-dimensional Co-culture system even without any chemical agents.
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KEYWORD
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bone marrow stromal cell(BMSCs), np-like cell, 3-dimensional co-culture
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